Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

Aim: The Phalaenopsis Orchid, also known as the Moth Orchid, is a magnificent multicolored big orchid that has become a popular indoor plant and is easily recognized nowadays. The study of identifying the effects of culture medium, growth regulators, and organic additives on shoot growth from regenerated hybrid-seeds of Phalaenopsis is required in the first three months.

Methodology: Shoots from hybrid-seeds of Phalaenopsis germination after 2 months were used as materials. Experiments were designed with the effects of nutrition mediums (MS and VW), BAP, and organic additives in Randomized Complete Block Design (CRD) via evaluation parameters on shoot growth (via number of leaves) and root growth (via root number and root length).

Results: In Experiment 1, after 12 weeks, the number of leaves per shoot on 1/2xMS medium increased from 3.45 to 4.50; and root growth performed better in 1/2xMS medium (4.06 cm). In experiment 2, 1/2xMS medium + 0.05 mg/L BAP showed the highest number of roots per shoot (4.80 roots per shoot). 1⁄2xMS medium and VW medium without BAP showed the longest roots length (4.06 cm and 4.18 cm, respectively). In experiment 3, VW medium + potato showed the highest number of leaves per shoot (8.95 leaves per shoot) and VW medium + coconut water (CW) showed the longest roots length (4.78 ± 0.09 cm). in experiment 4, root growth performed better in medium enriched with CW (4.80 roots per shoot and root length 2.69 cm).

Conclusion: Both 1/2xMS and VW medium are necessary for shoot from hybrid-seeds growth in the first 6-weeks and VW medium is favored for development of shoot and root after 6-weeks.