BIOSYNTHESIS AND CHARACTERIZATION OF LEAD SULFIDE NANOPARTICLES USING WASTEWATER BACTERIA

Many bacterial isolates were isolated from Zenine wastewater treatment plant, Giza, Egypt. The ability of the isolates to resist lead ions was studied. Serratia plymuthica was one of the most potent isolates that had the ability to resist lead ions at 80 ppm. the synthesize of lead nanoparticle by Serratia plymuthica was studied. Serratia plymuthica was grown aerobically and the cultures were challenged with Tryptic soy bean broth (TSB) containing 80 mg/l of Pb(NO3)2 in requisite ambience of laboratory. Cell lysate obtained, after the centrifugation of cultures were characterized through optical absorption by UV-Vis Spectrophotometer, X-ray Diffraction (XRD), Transmission Electron microscopy (TEM) and dynamic light scattering (DLS). The lead nanoparticles showed absorption peaks at ~ 330 nm corresponding to the Plasmon resonance lead nanoparticles (PbNPs). XRD Spectrum of these nanoparticles confirmed the formation of lead sulfide (PbS) particles by showing their respective characteristic peaks. TEM showed the production of these metal nanoparticles intracellularly and extracellularly. DLS results showed the ability of Serratia plymuthica to biosynthesis PbNPs with mean diameter 92.93 nm.