In-vitro Pharmacological Investigation of Ludwigia adscendens

Aims: This research objective was to investigate some in-vitro properties of methanol-extracted plant extracts of Ludwigia adscendens (the water primrose). The primary method of assessment for the methanolic extract of Ludwigia adscendens (MELA) was investigated for phytochemical screening. It was determined to look into the plant's potential for in vitro activities, such as antioxidant, antimicrobial, anti-inflammatory anti-arthritis, cytotoxic, thrombolytic and membrane stabilizing activity due to the pharmaceutical interest in its component parts.

Methodology: Phytochemical screening was performed utilizing a battery of test reagents. Antioxidant, antimicrobial, anti-inflammatory, anti-arthritic, cytotoxic, thrombolytic and membrane stabilizing activities were measured at various doses using the DPPH Free Radical Scavenging Assay, the Disk Diffusion Method, Egg Albumin Protein Denaturation Assay, the Inhibition of Protein Denaturation Assay, the Brine Shrimp Lethality Bioassay, Clot lysis assay and heat induced and hypotonic induced haemolysis method.

Results: The findings of research on MELA's phytochemical screening showed the presence of a wide range of various phytochemical components. The higher antioxidant activity of MELA is shown by its IC50 value of 85.76 µg/mL. Significant antibacterial action was shown by MELA. As compared to the standard ascorbic acid, this extract showed significantly greater anti-arthritic and anti-inflammatory effects. MELA exhibited much higher LC50 value which is 1.147 µg/mL than the typical vincristine-sulphate in a lethality test with brine shrimp. Comparatively speaking, this herb has quite powerful thrombolytic action. Significant membrane stabilizing action has also been shown by MELA.

Conclusion: To wrap it up, it is clear that this plants phytochemical can be used for wide range of drug discovery field due to its potent pharmacological actions.