Inhibitory Effect of Lactobacillus plantarum CQPC05 Isolated from Sichuan Pickles on Constipation

This study mainly investigated the influences of Lactobacillus plantarum CQPC05 (CQPC05), isolated from pickled vegetables, on mouse constipation caused by activated carbon water. 16S rDNA technology was used to identify the microorganism, and activated carbon was used to establish a mouse constipation model. After the mice received L. plantarum (109 CFU/mL) by gavage, small intestine tissue sections were collected. The serum indices and small intestine-related mRNA expression were obtained. A strain of L. plantarum was identified, and named CQPC05. Body weight and activated carbon progradation rate in mice of CQPC05 treatment groups were significantly higher than those in the control group, and the excretion time of the first tarry stool was earlier than that of the control group. The results of serum indices indicated that serum gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels in the CQPC05 treatment groups were significantly higher than those in the control group, while the somatostatin (SS) level was significantly lower. Compared to the constipation control group, the result of q-PCR demonstrated that CQPC05 could up-regulate the mRNA expression of c-Kit, SCF (stem cell factor), and GDNF (glial cell-derived neurotrophic factor) genes and down-regulate the expression of TRPV1 (transient receptor potential cation channel subfamily V member 1) and iNOS (inducible nitric oxide synthase). In conclusion, L. plantarum CQPC05 can effectively alleviate constipation, and has good probiotic potential and application value.