Effects of MAPKK Inhibitor PD98059 on Growth of Maize Seedling

Aims: Preliminary observations showed that 75 ìM PD98059 had a long-term effect on growth of maize seedlings. To verify and systemically analyze the effects of 75 ìM PD98059 on growth of maize seedlings, we designed and conducted this experiment.
Methodology: We recorded and analyzed the effects of 75 ìM PD98059 on growth of maize seedling during the first fourteen days. The growth traits were observed. The length, fresh weight, and dry weight of shoots or roots, and the root/shoot ratio of fourteen-day old seedlings were measured. Cutting analysis was conducted to analyze the effects of PD98059 on shoot growth.
Results: The shoot and root length of control showed about 1.38- and 1.5-fold longer than that of PD98059-treated seedlings, respectively. The shoot and root fresh weight of PD98059-treated seedlings declined to 80% and 79.4% of the control, respectively. The shoot and root dry weight of PD98059-treated seedlings declined to 68.3% and 69.8% of the control, respectively. PD98059 also decreased the length, fresh weight, and dry weight of cuttings.
Conclusion: PD98059 had a negative effect on the growth of maize seedlings and this effect was overall on both shoots and roots. The effect of PD98059 on shoot growth seemed to be not due to detrimental effects of PD98059 on roots.