Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission

Ma, Dong and Zheng, Bin and Liu, He-liang and Zhao, Yong-bo and Liu, Xiao and Zhang, Xin-hua and Li, Qiang and Shi, Wei-bo and Suzuki, Toru and Wen, Jin-kun and Lo, Cecilia W. (2020) Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission. PLOS Biology, 18 (8). e3000808. ISSN 1545-7885

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Abstract

Although dysregulation of mitochondrial dynamics has been linked to cellular senescence, which contributes to advanced age-related disorders, it is unclear how Krüppel-like factor 5 (Klf5), an essential transcriptional factor of cardiovascular remodeling, mediates the link between mitochondrial dynamics and vascular smooth muscle cell (VSMC) senescence. Here, we show that Klf5 down-regulation in VSMCs is correlated with rupture of abdominal aortic aneurysm (AAA), an age-related vascular disease. Mice lacking Klf5 in VSMCs exacerbate vascular senescence and progression of angiotensin II (Ang II)–induced AAA by facilitating reactive oxygen species (ROS) formation. Klf5 knockdown enhances, while Klf5 overexpression suppresses mitochondrial fission. Mechanistically, Klf5 activates eukaryotic translation initiation factor 5a (eIF5a) transcription through binding to the promoter of eIF5a, which in turn preserves mitochondrial integrity by interacting with mitofusin 1 (Mfn1). Accordingly, decreased expression of eIF5a elicited by Klf5 down-regulation leads to mitochondrial fission and excessive ROS production. Inhibition of mitochondrial fission decreases ROS production and VSMC senescence. Our studies provide a potential therapeutic target for age-related vascular disorders.

Item Type: Article
Subjects: Impact Archive > Biological Science
Depositing User: Managing Editor
Date Deposited: 01 Feb 2023 06:20
Last Modified: 22 Mar 2024 04:05
URI: http://research.sdpublishers.net/id/eprint/1645

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